NASA Headquarter's Office of Space Access and Technology oversaw the RLV program, which was managed by the RLV Office at NASA's Marshall Space Flight Center, located in Huntsville, Alabama. Responsibilities of other NASA Centers include: Johnson Space Center, Houston, Texas, guidance navigation and control technology, manned space systems, and health technology; Ames Research Center, Mountain View, CA., thermal protection system testing; Langley Research Center, Langley, Virginia, wind tunnel testing and aerodynamic analysis; and Kennedy Space Center, Florida, RLV operations and health management.

Lockheed Martin's industry partners in the X-33 program were: Astronautics, Inc., Denver, Colorado, and Huntsville, Alabama; Engineering & Science Services, Houston, Texas; Manned Space Systems, New Orleans, LA; Sanders, Nashua, NH; and Space Operations, Titusville, Florida. Other industry partners are: Rocketdyne, Canoga Park, California; Allied Signal Aerospace, Teterboro, NJ; Rohr, Inc., Chula Vista, California; and Sverdrup Inc., St. Louis, Missouri.